|
KMID : 0363219960340040608
|
|
Korean Journal of Dermatology
1996 Volume.34 No. 4 p.608 ~ p.615
|
|
|
The Effect of Ascorbic Acid, TGF-beta, and IFN-gamma on Collagen Synthesis in Three-Dimensional Culture of Normal Human Skin Fibroblast
|
|
김정애
정진호/문상은/윤상호/권오상/은희철
|
|
|
Abstract
|
|
|
Background :
@EN The classical in vitro method for researching the fibroblast metabolis is a mono layer culture system. But there are a lot of facts that the artifical influence of monolayer culture situations do not resemble the in vivo conditions. Recently
three-dimensional culture method of fibroblast in collagen gel was developed and in this system fibroblasts seem to behave like in vivo situations.
@ES Objective :
@EN So we examined the effects of ascorbic acid. TGF-β, and IFN-γon fibroblast in collagen gel culture system and then compared these effects with those in monolayer culture system. We also studied the regulatory mechanism of collagen gene by
ascorbic
acid. TGF-β, fand IFN-γ by Northern blot analysis in monolayer culture system.
@ES Methods :
@EN Using fibroblats of the neonatal foreskin, we measured the collagen synthesis after treatment of ascorbic acid, TGF-β, and IFN-γin three-dimensional culture and monolayer culture system. We also checked the change of mRNA of collagen after
treatment in monolayer culutre.
@ES Results :
@EN The relative levels of collagenase-digestible protein synthesis in the three-dimensional aeemed to be lower than those in the monolayer system. However. difference of fold increase was not statistically significant. The expression of collagen
mRNA
was increased after treatment of TGF-βand decreased after IFN-γ treatment.
@ES Conclusion :
@EN From these results. fibroblasts in the three-dimensional culture system mignt resemble the in vivo conditions. The moleoular level of re4gulation of collagen synthesis by TGF-β, and IFN-γ was thought to be transcriptional. The
three-dimensional
culture system could be a good model of culture system of fibroblast in studying of aging process or the role of cytokine in wound healing. (Kor J Dermatol 199;34(4) : 608~615)
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|